Backbone assignment with NMRPipe

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Hi I'm new to nmrPipe and I have a question about where to start assigning the backbone resonances for a recombinant protein. I managed to collect data on a bruker instrument on a labeled protein and I can get as far as converting the data, phasing the data in nmrpipe, displaying the slices in nmrDraw and doing the peak picking in three dimensions. I have the primary sequence but I'm a bit overwhelmed by number of programs that seem to do backbone assignments. I have a 2D HSQC, a 3D HNCACB and CACBCONH which Any suggestions on where to go next?

nmrpipe backbone 3DNMR

asked Nov 16 '16 at 14:16

David Onofrei
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Hi,

You can teach yourself just watching a few videos. https://www.youtube.com/playlist?list=PLmYqoarOFAZ-aInZ5uM2pV63e7yMSHSuZ

I recommend watching:

NMRFAM-SPARKY: Automated peak picking and validation strategy

NMRFAM-SPARKY: Automated assignment by PINE

You can download software packages from here: http://pine.nmrfam.wisc.edu/download_packages.html

Also, this literature may be helpful: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861749/

Best, Woonghee

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answered Nov 28 '16 at 16:37

Woonghee
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nmrpipe ×24
3DNMR ×6
backbone ×2

Asked: Nov 16 '16 at 14:16

Seen: 3,752 times

Last updated: Nov 28 '16 at 16:37

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