Please, if somebody wants to help me?! I need to get 1H spectrum with spin lock pulse on Bruker AV 600 instrument. Which pulse sequence and which parameters should be used, please? Thank you, thank you, Marijana from Croatia asked Jan 08 '14 at 01:49 gugica |
Hi Marijana, I was looking for the same thing and to my knowledge there is no simple 1D sequence with a spin lock filter. However it is easy to create: insert the lines 4u pl29:f1 (p29 ph5) after the excitation pulse of your desired 1D sequence. Change the power level on f1 back as necessary. Add the line "p29=d29" to the header. Add the line ph5=1 1 1 1 1 1 1 1 3 3 3 3 3 3 3 3 to the phase cycle. d29 is now the parameter to edit for your desired spin lock filter duration (typically up to 250ms). Adjust pl29 to the low power level of a TOCSY spin lock pulse (this is important!!). On our spectrometer for example the spin lock power level is attenuated by 16.8 dB relative to the high power pulse. Hope this helps a bit, all the best Brigitte answered Jan 13 '14 at 00:30 |