why we are seeing negative PREs in paramagnetic relaxation enhancement experiment?

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Hi NMR Wiki users,

I'm using two point method(Marius Clore and Junji Iwahara methods) to measure the PREs, but in my experiment I observed so many negative PREs. Is there any chance to use these negative PREs data in structure calculation.How can I get all positive PREs,otherwise I lose half of the data in my calculations.

pre

asked Dec 11 '12 at 07:48

Kris
11

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Asked: Dec 11 '12 at 07:48

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Last updated: Dec 11 '12 at 07:48

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