Hi, I am a new member to this community. I would like to set up an experiment, in which I have to set up proton carrier frequency at 7ppm during t1-evolution period and at water frequency during t3-direct detection period. How to do this? any suggestions? asked Aug 16 '10 at 05:55 kmd |
You can define a frequency list file in directory there you make a file containing:
e.g.
Once you have the file, you should put 'fq1' command in a pulseprogram (fq1:f1) and define the list file in acquisition menu under 'lists'. Check noediff, std-related pulseprograms in Bruker PP library. answered Aug 17 '10 at 12:52 MKarjuna hmm.. strange in lines breaks on example. - MKarjuna (Aug 17 '10 at 12:53) hey, thanks for stepping in, the "literal" formatting is indented by four spaces or can be inlined with backticks - Evgeny Fadeev (Aug 17 '10 at 12:56) the markup language used here is called markdown http://en.wikipedia.org/wiki/Markdown - Evgeny Fadeev (Aug 17 '10 at 12:57) thank you very much - kmd (Aug 19 '10 at 09:08) now I recall that you can specify offset for the shaped pulses separately using SPOFFS1 for sp1, etc. - Evgeny Fadeev (Aug 19 '10 at 10:27) |
Hi kmd, You'll need to set frequency offset before the "t1" excitation pulse to whatever corresponds to 7 ppm - that will cause "t1" evolution to be encoded relative to the frequency of that pulse. Similarly - to read relative to water resonance - set transmitter offset to the water offset value just before the readout pulse. If you have a Varian system you would do something like:
Hope that someone will explain how to do this for a Bruker system or any other details. answered Aug 16 '10 at 10:53 Evgeny Fadeev Thanks for the reply. I am working on a Bruker instrument. How to change the pulse sequence in that case. Thanks in advance - kmd (Aug 16 '10 at 11:56) |