Ok, on this particular Agilent MR400 we have a OneProbe with a ProTune module. It's presently set up and calibrated for 1H, 13C, 15N, F, and P. I would like to add 11B to the list. But we're currently lacking spectroscopist who could show me how to do it in the department.
I imagine there are 3 steps... Setup up ProTune for 11B, the calibrate the probe for 11B, and finally set up experiments for 11B. Unfortunately I don't really know how to do much on any of this and it's certainly not in the manual.
Where might I find resources for this?
asked Jul 16 '12 at 08:09
The extent to which you set the instrument up depends on how regularly you wish to take this data and whether you wish to incorporate the "Adaptive NMR" routines that automatically set the nt parameter depending on your sample concentration.
We've got this set up on our two 400MR-DD2's such that we: 1) ran some exploratory spectra to figure out appropriate paramters (e.g., we take advantage of 11B's short T1 and run d1=0.1, at=0.1, pw=(90 degree), and I think nt=1024 by default; I'd have to check on sw and tof. (With the VJ 3 software, you shouldn't need to "set up" 11B in Protune. Just click "Tune" in the Start/Standard panel and, if necessary, select B11 as the nucleus you want and go for it.) 2) added the 11B nucleus to our probefile, following the procedure documented in the VnmrJ 3.2 HTML online help 3) created a protocol button for 11B acquisition 4) set the system up so it will automatically set "nt" when provided a concentration; I used the clear instructions in the html VnmrJ help found when searching "adaptive nmr"
We now have people running 11B in automation all the time. Its fairly common to see a Study in which someone's queued up a 1H, a 13C, a 11B, a 1H-13C gHSQCAD, and all sorts of other experiments, all of which have nt set automatically, which is a huge benefit when you don't intuitively know how long it will take to get a good spectrum.
Would you like more details?
answered Jul 19 '12 at 05:54