Unanswered questions

dmf problem in vnmr

1 1 4k
answers votes views
Hi, dear all, I try to use garp1 sequence for decoupling with the statement " obsprgon("garp1", pw, angle, 0) ". If an...
Updated: Frey 61 Jun 30 '11 at 06:14

Converting from 2 column data to Topspin fid

1 1 4k
answers votes views
I am new to Topspin and would like to know if a 2 column dataset (containing time versus intensity) can be converted int...
Posted: Arvind Ananthanarayanan 11 / Updated: bernie o'hare 111 Aug 06 '11 at 10:48

Dimer and tetramer equilibrium identification of protein with NMR experiments

2 0 8k
answers votes views
Dear nmr wikiers could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
Posted: sri 71 / Updated: Justin Frank 1 Jun 29 '16 at 19:09

negative NMR peaks

1 0 11682
answers votes views
I am very new to NMR and have little experience of it. However I am trying to use it to determine the composition of a m...
Posted: Dean Woods 1 / Updated: Assma El 1 Jul 18 '11 at 01:58

Impact of deuteration on relaxation rates PROTEIN NMR

1 0 4k
answers votes views
DEAR NMR WIKIERS IS THEIR ANY NMR EXPERMENT TO KNOW THE IMPACT OF DEUTERATION ON RELAXATION REATES OF C13,N15,H ALFA , ...
Posted: sri 71 / Updated: Evgeny Fadeev 5771 Jun 21 '11 at 15:36

caluclation of duterium percentage of randomly deuterated protein

1 0 4k
answers votes views
Dear NMR WIKIERS , IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated prote...
Posted: sri 71 / Updated: Evgeny Fadeev 5771 Jun 21 '11 at 20:32

NMRPipe: re-integrate peaks

1 1 5k
answers votes views
Hello, I am familiarizing myself with NMRPipe and I have a question similar to the one already asked (http://qa.nmrwiki...
Updated: vvostri 11 Jun 14 '11 at 09:54

about sideband in solid-state NMR

2 1 12705
answers votes views
Hi, dear all, I've encounter a strange phenomemon. While the target speed of MAS was set to be 12kHz, the sidebands of ...
Posted: Frey 61 / Updated: Alexey Potapov 21 Jul 04 '11 at 10:17

Can correct TAUc be a sufficient evidence of the absence of aggregation?

2 0 7k
answers votes views
Hello! I am studying the protein backbone dynamics using the standard set of NMR relaxation parameters (R1, R2, NOE). ...
Posted: Andrey 1 / Updated: Yoan Monneau 136 Jul 01 '11 at 03:52

NMR resonance Lock

2 1 4k
answers votes views
I am running a varian gemini 300 on SUN microsystems with UNIX. I am trying to lock on my sample, and my lock is not sho...
Posted: MONOJON 11 / Updated: Jerry Hirschinger 199 Jun 16 '11 at 09:46

Methanol-d in chloroform-d

2 0 7k
answers votes views
Hi, This is a question related to the question I posted earlier titled "H NMR integration". I tried adding one drop of ...
Updated: Davis Chen 11 Jun 13 '11 at 20:39

Low resolution of pepitde samples

3 0 5k
answers votes views
Dear friends, I am now trying to record the NOESY spectrum of my 30 amino acids peptide on DRX600 with a TXI cryo probe...
Posted: Yun Wang 1 / Updated: Evgeny Fadeev 5771 Jun 07 '11 at 14:00

adiabatic decoupling

1 1 6k
answers votes views
Hi NMRwikiers, for measuring scalar couplings we use some HSQC pulse sequences modified for obtaining the coupling in F1...
Posted: ptrigomou 11 / Updated: ChemMJW 99 Jun 28 '11 at 12:50

software for 1D-2D spectra assignment for small molecules

2 1 7k
answers votes views
Please advice good software for 1D and 2D spectra assignment and structure elucidation, for small organic molecules. I'm...
Posted: VVK 61 / Updated: Evgeny Fadeev 5771 May 23 '11 at 10:54

Downfield carbon paired with a upfield proton

4 1 6k
answers votes views
Dear sirs and madams, I have a natural product whose two carbon resoances are appearing at 46 and 56 ppm but theirs prot...
Posted: Jonnala Kotesh Kumar 11 / Updated: nvgramosa 11 Oct 08 '11 at 09:08

Best Relaxation delay 3D NMR

1 1 6k
answers votes views
Dear NMR WIKIers I prefer to measure best relaxation delay for 3D nmr experments by giving array ( like 0.5 , 0.6 , 0....
Updated: sri 71 May 24 '11 at 02:44

Aminoacid residue identification or assignment of protein from 3D NMR Experments

3 1 6k
answers votes views
Dear NMR Wiki'ers I am identifying aminoacid residues or assignment of protein from following 3D NMR experments HNCA...
Posted: sri 71 / Updated: Justin Frank 1 Jun 29 '16 at 19:53

H NMR integraton

3 1 12389
answers votes views
Dear all: I just synthesized a polymer. The integrations in the high-chemical-shift regime are consistent with theoretic...
Posted: Davis Chen 11 / Updated: sekhar Talluri 621 Jun 02 '11 at 13:33

Learning pulse programming

1 1 5k
answers votes views
I'm a grad student with no prior experience in pulse programming. Currently I work in rodent MRI. I want to be able to...
Posted: seedy 11 / Updated: Yoan Monneau 136 May 14 '11 at 12:27

3DNMR CBCANH experment

5 1 9k
answers votes views
Dear NMR wiki ers Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL...
Updated: sri 71 Aug 25 '11 at 00:09

data processing

4 1 7k
answers votes views
HELLO NMR WIKI ERS Could you please give information about the standred reference values of LINEAR PRIDICTION FORWRAD...
Updated: sri 71 May 24 '11 at 02:45

assignment of 12 kd protein

6 1 7k
answers votes views
Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11

CT COSY pulse sequence programming

1 1 5k
answers votes views
Hi, I want to make a CT-COSY pulse sequence on a Brucker AVANCE II spectrometer. But I'm bother by incremented delay. W...
Posted: dimichu 11 / Updated: Clemens Anklin 416 May 07 '11 at 03:38

SSNMR 2H wideline probe that can access sample temperatures in the range of 20-150 K?

1 0 5k
answers votes views
Is anyone aware of NMR facilites, which possess a SSNMR equipped with a 2H wideline probe that can access sample tempera...
Posted: Cortnie Vogelsberg 21 / Updated: Alexey Potapov 21 Jul 04 '11 at 10:24

Generate inter-proton distance restraints from an X-ray structure

1 1 4k
answers votes views
Hello, Does anyone know of any program that can generate inter-proton distance restraints from an X-ray structure? I w...
Posted: Wenjin Wu 61 / Updated: Josef Chmelik 1 May 07 '13 at 05:33

Problem with both dispersed and absorbed peaks

1 1 5k
answers votes views
hi when i run homonuclear NOESY experiments in bruker 500MHz spectometer with d8 150ms(mw is 300).Though i am followi...
Updated: Ganesh 21 Apr 24 '11 at 23:21

Error 113: lid is close

1 1 6k
answers votes views
Was trying to lift sample in BMSB control suite... but the machine detected sample within and an error 113 popped out. A...
Posted: conny 11 / Updated: Jerry Hirschinger 199 Apr 19 '11 at 07:40

Chemical exchange of Tyr residues - what information can be found about dynamics?

1 1 6k
answers votes views
I have an aromatic spectrum of Tyr residues in p53 DNA binding domain. No peaks are seen for Tyr-205 at high temp, but a...
Posted: Amélie Wallenhammar 11 / Updated: Paul Driscoll 81 Apr 12 '11 at 04:30

incorrect processing of 2D HSQC in NMRPipe - what is wrong?

3 1 17297
answers votes views
i'm trying to process an HSQC by using standard scripts of NMRpipe, and get this: what is wrong in my processing? at...
Posted: nadia1230 11 / Updated: TJCarruthers 31 Apr 19 '11 at 16:10

1D projections in vnmrj, incorect slice?

0 1 2k
answers votes views
I have been trying to use the command 'proj' (in vnmrj 2.1) with a given frequencey and frequency range to do a sumn pro...
Posted: Thomas Garner 81 Apr 07 '11 at 11:00
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