i like this post (click again to cancel)
0
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

Recently we exchanged our old consoles (Avance III and DRX) with the Bruker’s new NEO series. After the installation of the old probes on the new consoles, I noticed that on the BBI probe, the HSQC experiment is no longer working – no signals. It seems the problem comes from the carbon decoupling pulse. This is very strange for me since this was my routine probe (for 5 years) and it worked perfectly on the Avance III console. Other experiments not using this decoupling pulse work very well. How can I check if this pulse was set properly? With TopSpin 4 it is calculated automatically in the prosol table. What is an optimal length 60-70 usec or 80 usec? If it is a problem with the probe, what can be suddenly damaged? Thanks, alina

asked Jan 29 '18 at 05:52

alina's gravatar image

alina
1

Be the first one to answer this question!
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

Tags:

×1

Asked: Jan 29 '18 at 05:52

Seen: 9,379 times

Last updated: Jan 29 '18 at 05:52

powered by CNPROG