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Hi,

which aminoacid is best for calibrating acrylamide gel percentage for RDC?

asked Dec 26 '13 at 02:17

shine%20a's gravatar image

shine a
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Deuterium (2H) signal splitting (in Hz) is usually measured using a 1D 2H NMR to 'calibrate' the extent of RDC, and therefore the gel percentage. Just for your reference.

link

answered Dec 29 '13 at 13:53

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dejian
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