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Ok, let's face it. I don't know what I'm doing. I'm a separations chemist running our NMR. sigh. Anyway, I have been completely unsuccessful in getting any 1D selective excitation experiments to work. 2D run beautifully and give the results I expect, but if I try and select a a peak that had noe/roe interactions, the 1D comes up totally blank. I've adjusted mixing times to be in the correct window for each peak that I've tried to observe. The probe has been recently calibrated for optimal pw etc. My biggest fear is that I'm going to have to crack open pbox and adjust or create new pulse shapes. VnmrJ 2.2D barely runs as it is (so finnicky and buggy!) that I haven't even started to try to learn pbox. Is there a good online tutorial or knowledge base to learn how to use it? I feel totally lost just looking at it. thanks! |
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Hello, you have to follow the "Varian/Agilent" procedures, to set it up correctly. There is a nice formula, how to do it practically: http://www.umich.edu/~chemnmr/docs/noesy1d.pdf ... pbox is very nice an clever, if you do one thing - "Never open the pandora's box!!!!"! pbox is the short form of ???? Varian's software does the shape "on the fly" via the "pbox" if you don't use the commands "go" or "ga"! Always click on the green button "Acquire", then the job of doing the shape calculations are done automatically! Normally, this works fine and smooth! Yours, Ulrich Haunz |
