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Most 90 degree pulse calibration instructions I've run across seem to be for a 1H 90. What about a 13C 90 degree pulse? Anyone have an easy set of instructions for doing this? Is calibrating the 90 for 13C less important than the 1H 90 for 2D experiments? Thanks in advance! |
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As an addition to the two given answers, there is also a method written in the book "200 and more nmr experiments" page 18. I like it as it is really fast and gives a good estimation of a 90° pulse length for 13C. Especially for people like me who don't have access to admin passwords for every device to set probe head settings and usually you can't find a lab technician around. I can write a short explanation here if you don not have access to the book. |
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And if you do not find the manual Kirk is mentioning try "1D and 2D Step-by-Step Basic". You will find this under Help (?) --> Manuals. Pulse calibration procedures are described in Chapter 8. |
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The procedures that you want will be found in Bruker's manual: "Acceptance Test Procedures for Avance NMR Systems". There should be a copy with your system. After you have calibrated the hard pulses, you need to fill them into the "prosol" table. You will normally only have to do the hard pulse calibration procedures, as the prosol editor will generally calculate all of the low level (decoupler) and shaped pulses for you. |
