3d Tocsy-hsqc mgnetization transfer problem

i like this post (click again to cancel)

i dont like this post (click again to cancel)

remove favorite mark from this question (click again to restore mark)

Dear All, I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature? Thank you in advance\

3dTocsyhsqcspin

asked Mar 06 '12 at 00:20

Mario Scrima
1

One Answer:

oldest newest most voted

i like this answer (click again to cancel)

i dont like this answer (click again to cancel)

Hi Mario,

Relaxation is probably doing you in - the small protein is in a 30+ kD detergent micelle (size varies with detergent, and somewhat with solution conditions), so T2's are too short for a long enough mixing time to get full spin system cross-peaks. I'd suggest backing off to a 40-48 msec mixing time to (hopefully) at least get the H-betas. - Mark

link

answered Mar 09 '12 at 07:39

Mark Girvin
11

Here (once you log in) you will be able to sign up for the periodic email updates about this question.

Your answer

Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)

toggle preview

Tags:

3dTocsyhsqcspin ×38

Asked: Mar 06 '12 at 00:20

Seen: 3,371 times

Last updated: Mar 09 '12 at 07:39

Related questions