N15 NNH separated NOESY

i like this post (click again to cancel)

i dont like this post (click again to cancel)

remove favorite mark from this question (click again to restore mark)

Dear friends,

I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get more information about this experiment from other sources. Is this experiment useful for providing restraints for protein structure calculation. what kind of information this experiment can provide and how to set up. If anyone has references to suggest, please suggest.

Thanks & Regards

Arun

noesy

asked Feb 21 '12 at 00:37

Arun Gupta
11

2 Answers:

oldest newest most voted

i like this answer (click again to cancel)

i dont like this answer (click again to cancel)

Hi Arun,

They might be referring to an HSQC-NOE-HSQC (or HMQC-NOE-HMQC, as originally described by Ikura et al JACS 112, 9020 (1990)). It's useful for identifying amide-amide NOEs when their proton chemical shifts are too close together. I think most people run it in its HSQC-NOE-HSQC form these days, or, for larger proteins as a 1H15N TROSY-NOE-TROSY. - Mark

link

answered Feb 21 '12 at 09:08

Mark Girvin
11

Guerry P, Duong V D, Herrmann T. CASD-NMR 2: robust and accurate unsupervised analysis of raw NOESY spectra and protein structure determination with UNIO[J]. Journal of biomolecular NMR, 2015, 62(4): 473-480.

Tzeng S R, Pai M T, Kalodimos C G. NMR studies of large protein systems[J]. Protein NMR Techniques, 2012: 133-140.

Shigemitsu Y, Ikeya T, Yamamoto A, et al. Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins[J]. Biochemical and biophysical research communications, 2015, 457(2): 200-205.

And some commercial platforms.

link

answered Jun 27 '16 at 22:53

Justin Frank
1

Here (once you log in) you will be able to sign up for the periodic email updates about this question.

Your answer

Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)

toggle preview

Tags:

noesy ×26

Asked: Feb 21 '12 at 00:37

Seen: 4,661 times

Last updated: Jun 27 '16 at 22:53

Related questions