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Dear All,

I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory.

Any suggestion would be useful

Regards

Arun

asked Feb 13 '12 at 07:04

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Arun Gupta
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Asked: Feb 13 '12 at 07:04

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