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Hi everybody! I have just started to work with TopSpin 3.1, though already faced a problem. I have an 15N-1H HSQC spectrum of one protein, the assignment of which has been done and deposited in BMRB. I want to import this assignment (the peaklist) into the Topspin, to be able to compare with another spectrum and analyse. Do I need to pick peaks and make first unassigned table and then import the assigned data? Is there any step by step instruction for that in Bruker? I was looking for it but couldn't find. I would appreciate any advice! P.S. It used to be simple and fast in CARA software, but i am stocked here in TopSpin.

asked Aug 22 '11 at 23:51

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elena
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First I made an automated picking and imposed the peaklist extracted from CARA into the TopSpin. But still, cross-peaks are still not assigned: in tab Peaks the Intensity column is filled with 0.00 values, though the Annotation column has the residue number and name. How can i solve it? - elena (Aug 23 '11 at 01:12)

You should be able to get intensity values loaded when you go to the peaks tab, right click the mouse and choose "reset intensities" and complete table. - Clemens Anklin (Sep 06 '11 at 11:54)

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Asked: Aug 22 '11 at 23:51

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Last updated: Sep 06 '11 at 11:54

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