Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. Is there a method to measure them anyway? Will E.COSY type of experiments still work?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. Is there a method to measure them anyway? Will E.COSY type of experiments still work?be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. protons (no discernible multiplet structure - even line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?samples? Pseudo-3D experiments?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) two-dimensional experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples? Pseudo-3D experiments?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!