How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it is likely that side-chains occupy many conformations.

However, the backbone is probably less mobile.

How do you calibrate NOE restraints based on the peak intensity in a situation like this?

Thank you.

How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it is likely that side-chains occupy many conformations.

However, the backbone is probably less mobile.

How do you calibrate NOE restraints based on the peak intensity in a situation like this?

Thank you.

How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it is likely that side-chains dynamically occupy many conformations.

However, the backbone is probably less mobile.

How do you calibrate NOE restraints based on the peak intensity in a situation like this?

Thank you.

How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it is likely that side-chains dynamically occupy many conformations.

However, the backbone is probably less mobile.

How do you calibrate NOE restraints based on the peak intensity in a situation like this?

edit: sorry I was not clear enough the first time - I mean that conformational exchange that is fast on NMR time scale.

Thank you.