Hello, what NMR experiments can detect hydrogen bonds directly?
Is there anything that can work with non-labeled samples at the natural abundance of isotopes?
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initial version
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Hello, what NMR experiments can detect hydrogen bonds directly?
Is there anything that can work with non-labeled samples at the natural abundance of isotopes?
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No.1 Revision
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Hello, what NMR experiments can detect hydrogen bonds directly?
Is there anything that can work with non-labeled samples at the natural abundance of isotopes?
We have a cyclic peptide that might form a beta-sheet made of two anti-parallel strands.
The problem is that it is made of two pieces of the same sequence closed into a cycle and in such arrangement NOE's from the "middle" part (if it is indeed forming two anti-parallel beta-strands) will be hard to distinguish as being intra- or inter-strand. That's why I think direct observation of hydrogen bonds would be very helpful.
Thanks!
