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posted Feb 26 '10 at 14:16

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry.

Also - I am interested in getting as much detail about the structure of the peptide as possible.

Do you typically need to record NOE buildup curves in such cases?

Given that side-chains of the peptide are unlikely to be packed like in the core of the globular protein - do you include side-chain NOE's into structure calculations?

Could you recommend any citations?

Thank you!.

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posted Feb 26 '10 at 14:16

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry.

Also - I am interested in getting as much detail about the structure of the peptide as possible.

Do you typically need to record NOE buildup curves in such cases?

Given that side-chains of the peptide are unlikely to be packed like in the core of the globular protein - do you include side-chain NOE's into structure calculations?

Could you recommend any citations?

Thank you!.

click to hide/show revision 3
No.2 Revision

posted Feb 26 '10 at 14:16

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry.

Also - I am interested in getting as much detail about the structure of the peptide as possible.

Do you typically need to record NOE buildup curves in such cases?

Given that side-chains of the peptide are unlikely to be packed like in the core of the globular protein - do you include side-chain NOE's into structure calculations?

Could you recommend any citations?

Thank you!.

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