Hello,

I work a lot with compounds containing numerous carbohydrate groups. Therefore I use frequently 1D-TOCSY experiment with varying mixing times (parameter d9 = 30, 60 and 120 ms for selmlgp pulse program). It would be very useful to have a pseudo-2D experiment with these 3 1D-TOCSY spectra saved as one ser file (I remember that it was possible on Varian instruments). We've got a Bruker 500 MHz apparatus with Avance III HD console. Topspin version 3.2 pl1. Can you help me with creating such experiment? Is it even possible? Regards, Luke

Hello,

I work a lot with compounds containing numerous carbohydrate groups. Therefore I use frequently 1D-TOCSY experiment with varying mixing times (parameter d9 = 30, 60 and 120 ms for selmlgp pulse program). It would be very useful to have a pseudo-2D experiment with these 3 1D-TOCSY spectra saved as one ser file.

<edit> Thanks to the help of Clemens Anklin from Bruker I was able to prepare an experiment with 'selmlgp.2' pulse program (1D TOCSY with supression of zero quantum coherence) modified by Clemens. You can download it here: selmlgpvd.2 Regards Luke Preparation (comments from Clemens): Define a 'vdlist' file (I remember that it was possible on Varian instruments). We've got a Bruker 500 MHz apparatus with Avance III HD console. Topspin version 3.2 pl1. Can in *\exp\stan\nmr\lists\vd\ directory with the various mixing time entries. Set TD1 equal to the number of entries and SI1 to the next nearest power of 2. Use the same parameters for processing in F2 as you help me with creating such experiment? Is it even possible? Regards, Lukewould use for the 1D's. Set FnMODE to QF. If you want to process each FID individually use the AU splitser to create 1D experiments. At the end of the acquisition use xf2 to transform all the spectra and then you can use the AU program split2D to generate individual 1D spectra.

Hello,

I work a lot with compounds containing numerous carbohydrate groups. Therefore I use frequently 1D-TOCSY experiment with varying mixing times (parameter d9 = 30, 60 and 120 ms for selmlgp pulse program). It would be very useful to have a pseudo-2D experiment with these 1D-TOCSY spectra saved as one ser file.

<edit> <edit> Thanks to the help of Clemens Anklin from Bruker I was able to prepare an experiment with 'selmlgp.2' pulse program (1D TOCSY with supression of zero quantum coherence) modified by Clemens. You can download it here: selmlgpvd.2 Regards Luke Preparation (comments from Clemens): Define a 'vdlist' file in *\exp\stan\nmr\lists\vd\ directory with the various mixing time entries. Set TD1 equal to the number of entries and SI1 to the next nearest power of 2. Use the same parameters for processing in F2 as you would use for the 1D's. Set FnMODE to QF. If you want to process each FID individually use the AU splitser to create 1D experiments. At the end of the acquisition use xf2 to transform all the spectra and then you can use the AU program split2D to generate individual 1D spectra.spectra. Regards Luke