Dear NMR wiki community,

I have recorded 24 sequential 15N HSQC spectra, in an H/D exchange experiment and now, I am having problem with the concept of what to use for the error bars on each spectrum signal intensity? To be as fast as possible, I have recorded the primary HSQCs only with 2 scans, later 4 scans, later 8.. I see, there is S/N ( 7 3 5 1 6 2 11 2 3 4 2 2 4 1 6 0 0 2 0 1 1 2 0 1)* and scans (2 2 2 4 4 4 4 8 8 8 8 8 8 8 16 16 16 16 16 16 16 16 32 32) and deviation.

• each experiment is separated by space

Probably, I have to correct the deviations manually if there is any but, how to combine S/N and number of scans to add error bars at each signal intensity?

I hope I am clear!

Thanks M.

Dear NMR wiki community,

I have recorded 24 sequential 15N HSQC spectra, in an H/D exchange experiment and now, I am having problem with the concept of what to use for the error bars on each spectrum signal intensity? To be as fast as possible, I have recorded the primary HSQCs only with 2 scans, later 4 scans, later 8.. I see, there is S/N ( 7 3 5 1 6 2 11 2 3 4 2 2 4 1 6 0 0 2 0 1 1 2 0 1)* and scans (2 2 2 4 4 4 4 8 8 8 8 8 8 8 16 16 16 16 16 16 16 16 32 32) and deviation.

• each experiment is separated by space

Probably, I have to correct the deviations manually if there is any but, how to combine S/N and number of scans to add error bars at each signal intensity?

I hope I am clear!

Thanks M.