Hellow,

I am trying to determine solution structure of a peptide in 50% TFE solution. I dissolved my peptide in 50% TFE-d3/50%H2O solution. When I enter "lock" on command table, I cannot find lock value on this solvent. (I am bruker 600 Hz NMR and using topspin 2.1 software). I tried to select TFE on the lock table, but the lock signal was wobbling too much and the line shape was not good. I tried to find lock value of 50% TFE on the internet, but I could not find. In this case, how should I do? I hope someone could me suggest any ideas about this.

Thank you very much.

Glenn

Hellow,

I am trying to determine solution structure of a peptide in 50% TFE trifluoroethanol(TFE) solution. I dissolved my peptide in 50% TFE-d3/50%H2O solution. When I enter "lock" on command table, I cannot find lock value on this solvent. (I am bruker 600 Hz NMR and using topspin 2.1 software). I tried to select TFE on the lock table, but the lock signal was wobbling too much and the line shape was not good. I tried to find lock value of 50% TFE on the internet, but I could not find. In this case, how should I do? I hope someone could me suggest any ideas about this.

Thank you very much.

Glenn

Hellow,Hello,

I am trying to determine solution structure of a peptide in 50% trifluoroethanol(TFE) solution. I dissolved my peptide in 50% TFE-d3/50%H2O solution. When I enter "lock" on command table, I cannot find lock value on this solvent. (I am bruker 600 Hz NMR and using topspin 2.1 software). I tried to select TFE on the lock table, but the lock signal was wobbling too much and the line shape was not good. I tried to find lock value of 50% TFE on the internet, but I could not find. In this case, how should I do? I hope someone could me suggest any ideas about this.

Thank you very much.

Glenn