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initial version
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Dear NMR wiki ers
Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion )
Iam always geting bad quality CBCANH experment on 700 vnmr cryob Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment
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No.1 Revision
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Dear NMR wiki ers
Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion )
Iam always geting bad quality CBCANH experment on 700 vnmr cryob Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment
