Dear memers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone using HNCA, HNCOCA CBCANH and CBCACONH , still some peaks are not quality of cbcanh and cbcaconh . how to assign rest of them using other experments ( HCCONH, HBHACONH ,CCCONH ) ? iam using sparky , vnmrj 700 mhz

thanking you anticipation

Dear memers NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone using HNCA, HNCOCA CBCANH and CBCACONH , still some peaks are not quality of cbcanh and cbcaconh . how to assign rest of them using other experments ( HCCONH, HBHACONH ,CCCONH ) ? iam using sparky , vnmrj 700 mhz

thanking Thanking you anticipation