Will intermediate exchange give poor half filtered NOE

I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would this be?

The interaction is in intermediate exchange, so presumably if my labelled protein is saturated to give me sharp peaks the partner will have begun to broaden out. Will the observed NOE suffer the same broadening or will it simply carry the chemical shift of its bound state? I thought it would also broaden but I am unsure of this.

Any help would be appreciated

Thanks

merged follow up with the original question

posted Jul 15 '10 at 08:17

Evgeny Fadeev
577●1

Will intermediate exchange give poor half filtered NOE

I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would this be?

Any help would be appreciated

Thanks

edit: My assumption was that broadened resonances due to exchange in one protein would cause the intermolecular NOE to broaded also, what I was unsure about is whether I was missing somthing and say, in reality because the intermolecular NOE was originating only from the bound state, i.e. one of the exchanging species, whether it would still be broad or maintain the chemical shift of the bound species from which the NOE is coming from

intermediate chemical-exchange complex noesy