Since my university does not have a professional spectroscopist, I am a new learner of NMR, and I really have no one else to ask...

I have a very concentrated sample (small molecule, MW < 300, 100 mg / mL), and I am running a 2D NOESY experiment (pulseprogram = noesyph in Bruker 400 MHz). However, the NOE signals that I am looking for are very weak, hidden by serious t1 noise (since the signal concerned is a CH3 group, very strong proton signal). Besides tricks like symmetrization, what else can I do to reduce the t1 noise or strengthen my NOE signal?

Shall I: 1. Increase ns (no of scan, currently ns = 8)? 2. Increase d1 (relaxation delay, currently d1 = 3 s)? 3. Increase d8 (mixing time, currently d8 = 1.5 s)?

Thanks a lot for your help!

Since my university does not have a professional spectroscopist, I am a new learner of NMR, and I really have no one else to ask...

I have a very concentrated sample (small molecule, MW < 300, 100 mg / mL), and I am running a 2D NOESY experiment (pulseprogram = noesyph in Bruker 400 MHz). However, the NOE signals that I am looking for are very weak, hidden by serious t1 noise (since the signal concerned is a CH3 group, very strong proton signal). Besides tricks like symmetrization, what else can I do to reduce the t1 noise or strengthen my NOE signal?

Shall I: 1. Increase ns (no of scan, currently ns = 8)? 2. Increase d1 (relaxation delay, currently d1 = 3 s)? 3. Increase d8 (mixing time, currently d8 = 1.5 s)?

Thanks a lot for your help!