hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2CH2= protons in CH2=CH- the acryl group are different, one is cis to CH, another one is trans.

And your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks per chain? Could you clarify that by editing your original question - which ratio are you after?

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

And Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks blocks of the two kinds per chain? Could you clarify that by editing your original question - which ratio are you after?

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks of the two kinds per chain? Could you clarify that by editing your original question - which ratio are you after?

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks of the two kinds per chain? Could you clarify that by editing your original question - which ratio are you after?

On the practical side - use small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals.integrals. If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails.

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks of the two kinds per chain? Could you clarify that by editing your original question - which ratio are you after?

On the practical side - use small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals. If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails.tails of the Lorentzian.

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks of the two kinds per chain? chain or the ratio of acryl/ethyleneglycol units? Could you clarify that by editing your original question - which ratio are you after?

On the practical side - use small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals. If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

hmm, trying to imagine the molecule you have. So what I understand you are looking at randomly repeated units like:

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

terminated at both ends with CH2=CH-C[=O]-O- moyeties? or is there CH3-CH[COOH]- termination? CH2= protons in the acryl group are different, one is cis to CH, another one is trans.

Is your goal is to determine average <n/m> - (average ratio of block lengths) or average ratio of number of blocks of the two kinds per chain or the ratio of acryl/ethyleneglycol units? Could you clarify that by editing your original question - which ratio are you after?

On the practical side - use small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals.

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

-(-CH2-CH[COOH]-)n-(-O-CH2-CH2-O-)m-

On the practical side - use small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals.

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side - use it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice) when you need accurate integrals. choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

...-M-CH2-C[O]-M-CH2-C[O]-...

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have one more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way?

...-M-CH2-C[O]-M-CH2-C[O]-...
...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have one more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have one two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>?

Maybe for your question #1 - the third "acrylate" peak is from PLA's CH?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>?

Maybe for your question #1 - the third "acrylate" peak is from PLA's CH?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way?way (that linker single acrylate is "extended" instead of popping the CH3 group?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>?? This part I just did not get - what ratio are you trying to determine?

Maybe the third "acrylate" peak is from PLA's CH?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group?group)?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>? This part I just did not get - what ratio are you trying to determine?

Maybe the third "acrylate" peak is from PLA's CH?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>? This part I just did not get from your original question - what ratio are you trying to determine?

Maybe the third "acrylate" peak is from PLA's CH?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>? This part I just did not get from your original question - what ratio are you trying to determine?

Maybe the third "acrylate" peak is from PLA's CH?

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...

Are you trying to calculate <x/m>? This part I just did not get from your original question - what ratio are you trying to determine?

Maybe the third "acrylate" peak is be from PLA's CH?

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH-C[O]-M-CH2-CH-C[O]-...
...-M-CH2-CH(COOH)-M-CH2-CH(COOH)-...

Are you trying to calculate <x/m>? This part I just did not get from your original question - what ratio are you trying to determine?

Maybe the third "acrylate" peak be from PLA's CH?

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH(COOH)-M-CH2-CH(COOH)-...

Are you trying to calculate <x/m>? This part I just did not get from your original question - what ratio are you trying to determine?

Maybe the third "acrylate" peak be from PLA's CH?

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios will work, but it's important that the spectrum is recorded so that integral values can be trusted. On the practical side it is better to small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH(COOH)-M-CH2-CH(COOH)-...

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios will work, but should work and it should be possible to determine for example

or

.

For the experiment to give correct integral ratios it's important to make sure that the spectrum is recorded so that integral values none of the resonances are even partially saturated. That can be trusted. On the practical side it is better to achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M- that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH(COOH)-M-CH2-CH(COOH)-...

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?

Yes, the integral ratios should work and it should be possible to determine for example

<p/x> or

<p/m>.

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Does your polymer consist of multiple macromers -M-Looks like the repeating unit is that you've described? Are they connected this way (that linker single acrylate is "extended" instead of popping the CH3 group)?

...-M-CH2-CH(COOH)-M-CH2-CH(COOH)-...

Also wouldn't there be three kinds of acrylate from PLA - those in the middle and those at the two edges, or at least two distinguishable ones?-(Acr)p-(PLA)x-(PEG)m-(PLA)x-

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>.

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Hello again, I think I understand your question better now, but still have two more:

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>.. After all the peaks are assigned :).

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Chemical shift of protons in CH2 of -O-CH2-CHX- should be around 4 ppm, I think.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Chemical shift of protons in CH2 of -O-CH2-CHX- should be around 4 ppm, I think.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a doublet and a singlet?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a doublet and a singlet?multiplet?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a multiplet?

Also, is it possible that certain conformation forces the protons in CH2 group become non-equivalent?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a multiplet?

Also, is it possible that certain conformation forces the protons in CH2 group become non-equivalent?

Finally I thought that in the cases where p=1 and p!=1 chemical shifts of acrylic residue will probably be different, no?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a multiplet?

Also, is it possible that certain conformation conformation forces the protons in CH2 group become non-equivalent?

Finally I thought that in the cases where p=1 and p!=1 chemical shifts of acrylic residue will probably be different, no?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.

Looks like the repeating unit is -(Acr)p-(PLA)x-(PEG)m-(PLA)x-.

Yes, the integral ratios should work and it should be possible to determine for example <p/x> or <p/m>. After all the peaks are assigned :).

How broad are the lines? Is it possible that you see three peaks because there should be a multiplet?

Also, is it possible that certain conformation forces the protons in CH2 group become non-equivalent?

Finally I thought that in the cases where p=1 and p!=1 chemical shifts of acrylic residue will probably be different, no?

For the experiment to give correct integral ratios it's important to make sure that none of the resonances are even partially saturated. That can be achieved by using small flip angle for the pulse - maybe 20 degrees and allow sufficient relaxation (5*T₁ is a good choice).choice). Figure 4.3 on p. 113 of Timothy Claridge "High Resolution NMR Techniques in Organic Chemistry" had a good illustration of that.

Being careful with the choice of recycling delay and pulse width will be especially important if spins of nuclei "whose" peaks the need to be integrated have substantially different T1 relaxation times, e.g. methyl vs methyne.

If the lines are wide and overlapping, line fitting may be required (for example in Origin or Matlab) to extract the integrals. Also - with lorentzian lineshapes integral reset points must be quite far from the peak because of the wide tails of the Lorentzian.