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posted Mar 03 '10 at 12:38

I checked the post. My processing script looks like: nmrPipe -in test.fid \ | nmrPipe -fn SOL \ | nmrPipe -fn SP -off .4 -end 0.98 -pow 2 -c 0.5 \ | nmrPipe -fn ZF -auto \ | nmrPipe -fn FT \ | nmrPipe -fn PS -p0 -8.8 -p1 0.0 -di \ | nmrPipe -fn EXT -left -sw \ | nmrPipe -fn TP \ | nmrPipe -fn SP -off 0.4 -end 0.98 -pow 2 -c 0.5 \ | nmrPipe -fn ZF -auto \ | nmrPipe -fn FT \ | nmrPipe -fn PS -p0 0.0 -p1 0.0 -di \ | nmrPipe -fn TP \ | nmrPipe -fn COADD -cList 1 0 -time -axis Y \ | nmrPipe -fn POLY -auto \ -verb -ov -out A.ft2 I chage cList to 0 1 to get the 2nd part. Things looks OK in nmrDraw, however, there is a 2nd copy of the protein spectrum that folds from the top to the bottom of the y axis. The other copy is centered, but condensed along the y-axis. I am not sure why there are 2 copies of the spectrum appearing (besided the IP, and AP ones). The spectral widths are set up properly for this protein, and the HSQC with the same sw looks normal (1 copy). Maybe this is something to do with using -complex for processing the y dimension, where I normally use rance-kay. Any ideas? Thanks.

No.1 Revision

posted Mar 03 '10 at 13:02

Evgeny Fadeev
577●1

I checked the post. My processing script looks like:

nmrPipe -in test.fid \ | nmrPipe -fn SOL \ | nmrPipe -fn SP -off .4 -end 0.98 -pow 2 -c 0.5 \ | nmrPipe -fn ZF -auto \ | nmrPipe -fn FT \ | nmrPipe -fn PS -p0 -8.8 -p1 0.0 -di \ | nmrPipe -fn EXT -left -sw \ | nmrPipe -fn TP \ | nmrPipe -fn SP -off 0.4 -end 0.98 -pow 2 -c 0.5 \ | nmrPipe -fn ZF -auto \ | nmrPipe -fn FT \ | nmrPipe -fn PS -p0 0.0 -p1 0.0 -di \ | nmrPipe -fn TP \ | nmrPipe -fn COADD -cList 1 0 -time -axis Y \ | nmrPipe -fn POLY -auto \ -verb -ov -out A.ft2A.ft2

I chage cList to 0 1 to get the 2nd part. Things looks OK in nmrDraw, however, there is a 2nd copy of the protein spectrum that folds from the top to the bottom of the y axis. The other copy is centered, but condensed along the y-axis. I am not sure why there are 2 copies of the spectrum appearing (besided the IP, and AP ones). The spectral widths are set up properly for this protein, and the HSQC with the same sw looks normal (1 copy). Maybe this is something to do with using -complex for processing the y dimension, where I normally use rance-kay. Any ideas? Thanks.