it seems to be while taking experiments , you kept wrong or mismatched spectral widths in f1, f2 , f3 axis usually HNCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCOCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCO CO - 20 ppm N - 40 ppm H - 14.1 PPM HNCACO CO - 20 ppm N - 40 ppm H - 14.1 PPM Just cross check with your experiments above spectral widths , if every thing fine , do synchronization (adjust ppm if find any difference still ) by using ST command in sparky .

it seems to be while taking experiments , you kept wrong or mismatched spectral widths in f1, f2 , f3 axis axis.

usually in HNCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCOCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCO CO - 20 ppm N - 40 ppm H - 14.1 PPM HNCACO CO - 20 ppm N - 40 ppm H - 14.1 PPM Just cross check with your experiments above spectral widths , if every thing fine , do synchronization (adjust ppm if find any difference still ) by using ST command in sparky .

Regards Srinivas

it seems to be while taking experiments , you kept wrong or mismatched spectral widths in f1, f2 , f3 axis.

usually in we use following spectral widths HNCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCOCA C13 - 30 ppm N - 40 ppm H - 14.1 PPM HNCO CO - 20 ppm N - 40 ppm H - 14.1 PPM HNCACO CO - 20 ppm N - 40 ppm H - 14.1 PPM Just cross check with your experiments above spectral widths , if every thing fine , do synchronization (adjust ppm if find any difference still ) by using ST command in sparky .

Regards Srinivas