could you please tell to us how much concentration of protein and drug u r taking ? and buffer conditions ?

I am not sure but i suspecting following property might be the reason 1. reasons

1. paramagnetic impurity concentration or viscosity of the sample increasing with ligand concentration .
2. ligand unspecified binding to protein .
3. ligand (highly basic or acidic nature)
2.
4. At higher concentration of ligand ( if u taken it in salt conditions )

could you please tell to us how much concentration of protein and drug u r taking ? and buffer conditions ?

I am not sure but i suspecting following property might be the reasons

1. paramagnetic impurity concentration or viscosity of the sample increasing with ligand concentration .
2. ligand unspecified binding to protein .
3. ligand (highly basic or acidic nature)
4. At higher concentration of ligand ( if u taken it in salt conditions )) it dissipates the radio frequency pulse.

could you please tell to us how much concentration of protein and drug u r taking ? and buffer conditions ?

I am not sure but i suspecting following property might be the reasons

1. paramagnetic impurity concentration or viscosity of the sample increasing with ligand concentration .
2. ligand unspecified binding to protein .
3. because of ligand (highly highly basic or acidic nature) nature effects on sample conditions .
4. At higher concentration of ligand ( if u taken it in salt conditions ) it dissipates the radio frequency pulse.

could you please tell to us how much concentration of protein and drug u r taking ? and buffer conditions ?

I am not sure but i I am suspecting following property might be the reasons

1. paramagnetic impurity concentration or viscosity of the sample increasing with ligand concentration .
2. ligand unspecified binding to protein .
3. because of ligand highly basic or acidic nature effects on sample conditions .
4. At higher concentration of ligand ( if u taken it in salt conditions ) it dissipates the radio frequency pulse.

could you please tell to us how much concentration of protein and drug u r taking ? and buffer conditions ?

I am not sure but I am suspecting following property might be the reasons

1. paramagnetic impurity concentration or viscosity of the sample increasing with ligand concentration .
2. ligand unspecified binding to protein .
3. because of ligand highly basic or acidic nature effects on sample conditions condition .
4. At higher concentration of ligand ( if u taken it in salt conditions ) it dissipates the radio frequency pulse.