Here is the link to the original paper (I'll update this answer once I read . A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply gaussian pulse like that for selective excitation the article).closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

Here is the link to the original paper. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

Here is the link to the original paper. for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give details below), there is G3(consists of three gaussian pulses back-to back and is used for inversion), G4 (four pulse cascade used for the excitation).

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give details below), there is G3(consists of three gaussian pulses back-to back and is used for inversion), G4 (four pulse cascade used for the excitation).

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

Gaussian pulse can also be used for selective saturation^[2], (ref), where a train of pulses separated by a short delay is applied for some period of time.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give details hopefully the real answer below), there is are also G3(consists of three gaussian pulses back-to back and is used for inversion), inversion) and G4 (four pulse cascade used for the excitation).excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

Gaussian pulse can also be used for selective saturation(ref), where a train of pulses separated by a short delay is applied for some period of time.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation(ref), where a train of pulses separated by a short delay is applied for some period of time.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offests offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) inversion) and G4 (four pulse cascade used for the excitation) excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation(ref), where a train of pulses separated by a short delay is applied for some period of time.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation(ref), where a train of pulses separated by a short delay is applied for some period of time.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation(ref), ) (which is applied for detection of binding of small molecules to large bio-molecules in STD experiments) , where a train of pulses separated by a short delay is applied for some period of time.time. Here selectivity will depend on the total duration of saturation time and parameters of the pulse, but it should be easy to run an STD-type of experiment with a small molecule alone first to make sure that saturation does not affect it.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation(ref) (which is applied for detection of binding of small molecules to large bio-molecules in STD experiments) , where a train of pulses separated by a short delay is applied for some period of time. Here selectivity will depend on the total duration of saturation time and parameters of the pulse, but it should be easy to run an STD-type of experiment with a small molecule alone first to make sure that saturation does not affect it.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3(consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective selective saturation (ref2,4) (which is applied for detection of binding of small molecules to large bio-molecules in STD experiments) , where a train of pulses separated by a short delay is applied for some period of time. Here selectivity will depend on the total duration of saturation time and parameters of the pulse, but it should be easy to run an STD-type of experiment with a small molecule alone first to make sure that saturation does not affect it.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.
4. Saturation transfer difference (wiki page)
5. Signal saturation in NMR (wiki page)

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3 (consists of three gaussian pulses back-to back and is used for inversion) and G4 (four pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation (2,4) (which is applied for detection of binding of small molecules to large bio-molecules in STD experiments) , where a train of pulses separated by a short delay is applied for some period of time. Here selectivity will depend on the total duration of saturation time and parameters of the pulse, but it should be easy to run an STD-type of experiment with a small molecule alone first to make sure that saturation does not affect it.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.
4. Saturation transfer difference (wiki page)
5. Signal saturation in NMR (wiki page)

The answer depends on which Gaussian pulse is in use. There is a simple gaussian (for which I give hopefully the real answer below), there are also G3 (consists of three different gaussian pulses back-to back and is used for inversion) and G4 (four pulse (four-pulse cascade used for the excitation) pulses. Cascade pulses have excitation or inversion profiles closer to rectangular.

Gaussian pulse can also be used for selective saturation (2,4) (which is applied for detection of binding of small molecules to large bio-molecules in STD experiments) , where a train of pulses separated by a short delay is applied for some period of time. Here selectivity will depend on the total duration of saturation time and parameters of the pulse, but it should be easy to run an STD-type of experiment with a small molecule alone first to make sure that saturation does not affect it.

Setting up single gaussian pulse

Here is the link to the original paper for a single gaussian pulse. A 10ms gaussian pulse calibrated for 90 degree on-resonance flip angle will introduce almost no excitation at offsets >300 Hz.

So if you apply a single gaussian pulse like that for selective excitation the closest other resonance must be farther than 300 Hz away. If you need better selectivity - then just proportinately increase duration of the pulse and re-calibrate it again so that it has 90 degree flip.

References

1. Christopher Bauer, Ray Freeman, Tom Frenkiel, James Keeler and A. J. Shaka Journal of Magnetic Resonance (1984) 58(3), 442-457
2. Moriz Mayer, Bernd Meyer. J. Am. Chem. Soc. 2001, 123, 6108-6117
3. Lyndon Emsley and Geoffrey Bodenhausen. Chem. Phys. Lett. 165(6), 469-476.
4. Saturation transfer difference (wiki page)
5. Signal saturation in NMR (wiki page)