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posted Aug 22 '14 at 20:38

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ChemMJW
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Do I understand you correctly that you're trying to find a single buffer system that spans a wide pH range? To do that, you'll definitely need to consider buffers with polyprotic acid components. However, why limit yourself to a single buffer composition. It's very common to screen the spectra in your protein using a wide variety of buffers to see which yield high quality spectra. As a starting point, take a look at this web page from the University of Buffalo: http://www.nmr2.buffalo.edu/nesg.wiki/Buffer_optimization There they give series of common buffers that span a large pH range. It's easy enough to buffer-exchange your protein and then record a quick spectrum. Since you're doing 31P NMR, simply exclude those buffers that contain phosphorous if you don't want to worry about extra signals appearing in your spectrum. good luck.
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posted Aug 22 '14 at 20:39

ChemMJW's gravatar image

ChemMJW
99

Do I understand you correctly that you're trying to find a single buffer system that spans a wide pH range? To do that, you'll definitely need to consider buffers with polyprotic acid components.

However, why limit yourself to a single buffer composition. It's very common to screen the spectra in your protein using a wide variety of buffers to see which yield high quality spectra.

As a starting point, take a look at this web page from the University of Buffalo:

http://www.nmr2.buffalo.edu/nesg.wiki/Buffer_optimization

There they give series of common buffers that span a large pH range. It's easy enough to buffer-exchange your protein and then record a quick spectrum. Since you're doing 31P NMR, simply exclude those buffers that contain phosphorous if you don't want to worry about extra signals appearing in your spectrum. good luck.

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posted Aug 22 '14 at 20:40

ChemMJW's gravatar image

ChemMJW
99

Do I understand you correctly that you're trying to find a single buffer system that spans a wide pH range? To do that, you'll definitely need to consider buffers with polyprotic acid components.

However, why limit yourself to a single buffer composition. composition? It's very common to screen the spectra in of your protein using in a wide variety of buffers to see which yield high quality spectra.

As a starting point, take a look at this web page from the University of Buffalo:

http://www.nmr2.buffalo.edu/nesg.wiki/Buffer_optimization

There they give series of common buffers that span a large pH range. It's easy enough to buffer-exchange your protein and then record a quick spectrum. Since you're doing 31P NMR, simply exclude those buffers that contain phosphorous if you don't want to worry about extra signals appearing in your spectrum. good luck.

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