Tagged questions

assignment of 12 kd protein

6 1 7k
answers votes views
Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11

Line broadening in nmr

5 0 8k
answers votes views
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small mol...
Posted: fatima A 1 / Updated: Evgeny Fadeev 5771 Oct 12 '11 at 07:25

peak missing in nh hsqc protein nmr

4 0 19096
answers votes views
Dear NMR wikiers I am assigning 95 aminoacid residue protein . I can see 76 cross peaks in N H HSQC on 700 vnmr cryob ...
Updated: sri 71 Aug 25 '11 at 00:05

Spectra from BMRB Chemical shifts

4 0 8k
answers votes views
Is it possible to generate a spectrum from BMRB chemical shifts? I want to generate a spectrum to use as a reference fo...
Posted: Andreas 1 / Updated: delaglio 1361 Dec 11 '11 at 14:15

NMR of proteins

3 0 6k
answers votes views
I need informations about nuclear magnetic resonance of proteins with figures. ...
Posted: f_tahoori 6 / Updated: Justin Frank 1 Jun 27 '16 at 20:47

NMR experiments to assign aromatic sidechains?

3 1 9k
answers votes views
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted: anita 31 / Updated: roseyp 46 Feb 22 '11 at 09:38

Aromatic sidechain assignment pulse sequences for Varian?

3 0 5k
answers votes views
Hi, someone asked a similar question before, but I'd like to know: Are there readily available varian pulse sequences t...
Posted: Magnethead 42 / Updated: Dummy 31 Nov 19 '10 at 06:28

Best pulse sequence for HSQC in Guanidine-HCl

3 1 8k
answers votes views
Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 401 Oct 30 '10 at 06:29

RDC gel preparation

3 0 7k
answers votes views
I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
Posted: andrewL 1 / Updated: Andrew Fowler 96 Apr 24 '13 at 07:06

Please suggest model proteins and peptides for NMR

2 1 5k
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Markus Seeliger 11 Aug 17 '10 at 13:16

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 13137
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Posted: Evgeny Fadeev 5771 / Updated: Justin Frank 1 Jun 27 '16 at 19:27

What is your test for the feasibility of protein structure determination?

2 1 6k
answers votes views
How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 5771 / Updated: Ilango 171 Jul 21 '10 at 12:35

Dimer and tetramer equilibrium identification of protein with NMR experiments

2 0 8k
answers votes views
Dear nmr wikiers could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
Posted: sri 71 / Updated: Justin Frank 1 Jun 29 '16 at 19:09

Protein-peptide binding NMR Experments

2 0 7k
answers votes views
Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Posted: sri 71 / Updated: Justin Frank 1 Jun 27 '16 at 03:02

selecting for side chains

2 1 3k
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Justin Frank 1 Jun 29 '16 at 19:22

1D NOESY with selective excitation and water suppression

2 0 10378
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
Posted: slawojek 1 / Updated: kyleabeauchamp 1 Mar 06 '14 at 15:37

Streaks in RDC Gel Spectrum

2 0 8k
answers votes views
Hello all, I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
Updated: ChemMJW 99 Dec 06 '13 at 19:10

How to adjust the receiver gain in a deuterium oxide protein sample?

2 0 7k
answers votes views
I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives...
Updated: norsouth 3 Dec 02 '13 at 11:16

How to assign (perturbed) resonances when structure is known?

1 0 5k
answers votes views
I had two problems like this one recently: have protein (in solution) of known general structure resonance assignments...
Posted: Evgeny Fadeev 5771 / Updated: Aleksandr Yakimov 11 Apr 25 '10 at 01:17

What is the minimum information required to calculate a structure with cyana?

1 1 8k
answers votes views
Hi guys : I have several spectra, and wanted to test out what data I do , and do not need, in order to effectively get a...
Posted: j 131 / Updated: Bharathwaj 112 Jun 28 '10 at 19:43

When to label peptides and proteins and when not?

1 0 9k
answers votes views
When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Posted: Evgeny Fadeev 5771 / Updated: Paul Driscoll 81 Feb 02 '11 at 04:28

Examples of protein degradation eg HSQC?

1 1 5k
answers votes views
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look ...
Posted: madge 11 / Updated: Evgeny Fadeev 5771 Aug 08 '10 at 16:13

cs rosetta compilation for linux 32

1 1 5k
answers votes views
Hi guys : I was wondering if anyone knows where I can find CS-Rosetta compiled for 32 bit linux..... Im having trouble ...
Posted: j 131 / Updated: Evgeny Fadeev 5771 Jan 13 '11 at 12:27

caluclation of duterium percentage of randomly deuterated protein

1 0 4k
answers votes views
Dear NMR WIKIERS , IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated prote...
Posted: sri 71 / Updated: Evgeny Fadeev 5771 Jun 21 '11 at 20:32

c13 filter noesy experment for homodimer protein

1 0 4k
answers votes views
Dear NMR wikiers I would like to take c13 filter noesy experment ( this experment explore residues of the interface of...
Updated: sri 71 Jul 05 '11 at 10:56

Buffer systems for protein pH titrations

1 0 8k
answers votes views
Hi All, I am trying to design a 1D 31P pH titration experiment for a protein. I have been trying to identify a good buf...
Posted: K Sippel 1 / Updated: ChemMJW 99 Aug 22 '14 at 20:40

Protein Backbone assignment

1 0 4k
answers votes views
There are many Software Tools available for that purpose. What are the problems there (if there are some)? Do you think...
Updated: FAS 1 Dec 08 '16 at 07:53

2D pulse sequences for NMR of protein/peptide complexes?

0 1 3k
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Jul 26 '10 at 09:25

csrosetta server application

0 0 1k
answers votes views
I upload my protein on csrosetta server for generating structures. I have provided TALOS data file. Its already two days...
Posted: anup 1 Jun 04 '13 at 06:09
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