Buffer systems for protein pH titrations

1 0 8k
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Hi All, I am trying to design a 1D 31P pH titration experiment for a protein. I have been trying to identify a good buf...
Posted: K Sippel 1 / Updated: ChemMJW 99 Aug 22 '14 at 20:40

Chemical shift

1 0 2k
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Hi there, I am seeing four signals at equal height between -0.6 and 0.5 PPM of a 1H NMR sample in dmso-d6. The reaction ...
Posted: TBS 1 / Updated: Eugene DeRose 41 May 23 '14 at 13:02

simulating 2D-NOESY spectrum from structural model

1 0 6k
answers votes views
Hello everyone Our research group planning to study metal-ion induced structural transition using NMR. We are new to thi...
Posted: karthik 1 / Updated: Thomas 1 May 19 '14 at 13:03

bruker s1 sortier

0 0 4k
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Hello, I can't get results, as unknown error occures data/results.csv I'd restarted spectrometr several time, but the s...
Posted: Konservator 1 / Updated: Jerry Hirschinger 199 May 06 '14 at 07:37

extract error bars from sparky S/N or deviation or both

0 0 2k
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Dear NMR wiki community, I have recorded 24 sequential 15N HSQC spectra, in an H/D exchange experiment and now, I am ha...
Updated: Maryam 1 Apr 14 '14 at 12:57

RGA setting too high on Bruker Avance 300

3 0 6k
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I'm using Bruker Avance 300 spectrometer (Topspin processing software). When using "RGA" GR value jumps up to 3000 and m...
Posted: Avance300 1 / Updated: John Hollerton 96 Sep 10 '14 at 04:07

Signal intensity affected by long-range proton coupling?

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Hi, I'm looking for a theoretical explanation for the effect of long-range (proton) couplings on the signal intensity of...
Updated: doodlebugs 1 Apr 14 '14 at 06:45

195Pt experiment setup help needed

4 0 7k
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We just bought a new console (with Topspin 3.2)for our Bruker 400 and noticed that not all experiments that we previousl...
Posted: IA 1 / Updated: Kirk Marat 711 Apr 04 '14 at 13:48

simulate 3D NOESY

1 0 4k
answers votes views
Hello everyone I am using NMRPipe to simulate 3D, noise-free NOESY spectra (13C or 15N resolved) from peak lists, but I...
Updated: canavanin 17 Mar 19 '14 at 05:17

Total t1 evolution time, TROSY, PRE distance calculation

0 0 3k
answers votes views
Hi all, This is my first post to the forum and I am admittedly new to the field of protein NMR. I appreciate your patie...
Posted: Logan 1 Mar 11 '14 at 09:04
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