Tagged questions

How to adjust the receiver gain in a deuterium oxide protein sample?

2 0 525
answers votes views
I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives...
Updated: norsouth 3 Dec 02 '13 at 11:16

Streaks in RDC Gel Spectrum

2 0 495
answers votes views
Hello all, I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
Updated: ChemMJW 101 Dec 06 '13 at 19:10

1D NOESY with selective excitation and water suppression

2 0 808
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
Posted: slawojek 1 / Updated: kyleabeauchamp 1 Mar 06 at 15:37

csrosetta server application

0 0 291
answers votes views
I upload my protein on csrosetta server for generating structures. I have provided TALOS data file. Its already two days...
Posted: anup 1 Jun 04 '13 at 06:09

selecting for side chains

1 1 646
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Matthew Revington 41 Jun 06 '12 at 10:34

RDC gel preparation

3 0 1k
answers votes views
I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
Posted: andrewL 1 / Updated: Andrew Fowler 96 Apr 24 '13 at 07:06

Spectra from BMRB Chemical shifts

4 0 2k
answers votes views
Is it possible to generate a spectrum from BMRB chemical shifts? I want to generate a spectrum to use as a reference fo...
Posted: Andreas 1 / Updated: delaglio 1361 Dec 11 '11 at 14:15

Protein-peptide binding NMR Experments

1 0 1k
answers votes views
Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Updated: sri 71 Oct 31 '11 at 12:20

Line broadening in nmr

5 0 2k
answers votes views
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small mol...
Posted: fatima A 1 / Updated: Evgeny Fadeev 5471 Oct 12 '11 at 07:25

c13 filter noesy experment for homodimer protein

1 0 1k
answers votes views
Dear NMR wikiers I would like to take c13 filter noesy experment ( this experment explore residues of the interface of...
Updated: sri 71 Jul 05 '11 at 10:56

peak missing in nh hsqc protein nmr

4 0 3k
answers votes views
Dear NMR wikiers I am assigning 95 aminoacid residue protein . I can see 76 cross peaks in N H HSQC on 700 vnmr cryob ...
Updated: sri 71 Aug 25 '11 at 00:05

Dimer and tetramer equilibrium identification of protein with NMR experiments

1 0 1k
answers votes views
Dear nmr wikiers could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
Updated: sri 71 Jul 05 '11 at 00:13

caluclation of duterium percentage of randomly deuterated protein

1 0 1k
answers votes views
Dear NMR WIKIERS , IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated prote...
Posted: sri 71 / Updated: Evgeny Fadeev 5471 Jun 21 '11 at 20:32

assignment of 12 kd protein

6 1 3k
answers votes views
Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11

cs rosetta compilation for linux 32

1 1 2k
answers votes views
Hi guys : I was wondering if anyone knows where I can find CS-Rosetta compiled for 32 bit linux..... Im having trouble ...
Posted: j 121 / Updated: Evgeny Fadeev 5471 Jan 13 '11 at 12:27

Best pulse sequence for HSQC in Guanidine-HCl

3 1 2k
answers votes views
Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 326 Oct 30 '10 at 06:29

Aromatic sidechain assignment pulse sequences for Varian?

3 0 2k
answers votes views
Hi, someone asked a similar question before, but I'd like to know: Are there readily available varian pulse sequences t...
Posted: Magnethead 42 / Updated: Dummy 31 Nov 19 '10 at 06:28

When to label peptides and proteins and when not?

1 0 2k
answers votes views
When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Posted: Evgeny Fadeev 5471 / Updated: Paul Driscoll 81 Feb 02 '11 at 04:28

What is your test for the feasibility of protein structure determination?

2 1 2k
answers votes views
How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 5471 / Updated: Ilango 161 Jul 21 '10 at 12:35

Examples of protein degradation eg HSQC?

1 1 1k
answers votes views
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look ...
Posted: madge 11 / Updated: Evgeny Fadeev 5471 Aug 08 '10 at 16:13

2D pulse sequences for NMR of protein/peptide complexes?

0 1 1k
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 21 / Updated: Evgeny Fadeev 5471 Jul 26 '10 at 09:25

What is the minimum information required to calculate a structure with cyana?

1 1 2k
answers votes views
Hi guys : I have several spectra, and wanted to test out what data I do , and do not need, in order to effectively get a...
Posted: j 121 / Updated: Bharathwaj 112 Jun 28 '10 at 19:43

How to assign (perturbed) resonances when structure is known?

1 0 2k
answers votes views
I had two problems like this one recently: have protein (in solution) of known general structure resonance assignments...
Posted: Evgeny Fadeev 5471 / Updated: Aleksandr Yakimov 11 Apr 25 '10 at 01:17

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

1 1 2k
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Updated: Evgeny Fadeev 5471 Mar 08 '10 at 09:45

NMR experiments to assign aromatic sidechains?

3 1 3k
answers votes views
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted: anita 31 / Updated: roseyp 46 Feb 22 '11 at 09:38

NMR of proteins

2 0 2k
answers votes views
I need informations about nuclear magnetic resonance of proteins with figures. ...
Posted: f_tahoori 6 / Updated: Paul Driscoll 81 Oct 06 '11 at 09:24

Please suggest model proteins and peptides for NMR

2 1 2k
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Markus Seeliger 11 Aug 17 '10 at 13:16
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