Tagged questions

simulate 3D NOESY

1 0 1k
answers votes views
Hello everyone I am using NMRPipe to simulate 3D, noise-free NOESY spectra (13C or 15N resolved) from peak lists, but I...
Updated: canavanin 17 Mar 19 at 05:17

selective noesy

5 0 1k
answers votes views
Hi, I have question for you who have Bruker instruments! On 300 MHz I worked on selective noesy pulse sequence (selnogp)...
Posted: gugica 1 / Updated: Pete Gierth 326 Feb 13 at 14:58

1D NOESY with selective excitation and water suppression

2 0 1k
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
Posted: slawojek 1 / Updated: kyleabeauchamp 1 Mar 06 at 15:37

How to start experiment at particular row

1 0 754
answers votes views
I was running a 3D 15N edit NOESY (on a Bruker 600) and it was inadvertently stopped. I would like to restart the experi...
Posted: Lola Brown 1 / Updated: Pete Gierth 326 Jun 13 '13 at 11:05

Difference between 13C edited and 13C resolved NOESY

1 0 752
answers votes views
Hii, I want to know the difference between 13C edited and 13C resolved NOESY and also tell me about the applicati...
Posted: ashu 1 / Updated: sekhar Talluri 601 May 25 '13 at 12:30

Calculating NOESY intensities from X-ray crystal structure

0 0 512
answers votes views
Hi folks, Has anyone calculated NOESY intensities from a X-ray crystal structure? CORMA program calculates. But have an...
Posted: nmrspin 1 May 10 '13 at 11:39

Why do we turn off sample spinning when we do NOESY experiment?

2 1 1k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5571 Jun 03 '12 at 17:23

"quiet" NOESY

1 0 1k
answers votes views
How to set "quiet" NOESY experiment on spectrometer Bruker ? ...
Posted: ilya khodov 1 / Updated: David O'Brien 1 Apr 25 '12 at 08:38

N15 NNH separated NOESY

1 0 1k
answers votes views
Dear friends, I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get ...
Posted: Arun Gupta 11 / Updated: Mark Girvin 11 Feb 21 '12 at 09:08

Isotope Filtered NOESY

1 0 2k
answers votes views
I'm an NMR newbie. I know isotope filtering can be used to study a complex under slow exchange, but for fast-exchange, i...
Posted: Student Tim 1 / Updated: Eugene DeRose 41 Feb 28 '12 at 12:56

Folding vs. Aliasing (in NOESY spectra)

1 0 2k
answers votes views
Hi everyone Does anyone happen to know if/how I can tell whether peaks outside the spectral width were either folded or...
Posted: canavanin 17 / Updated: Kirk Marat 656 Feb 14 '12 at 13:38

Problem while genrating the constraints form N-15 edit AND C-13 EDIT NOESY files

1 0 1k
answers votes views
I am encountering the problem while generating the constraints form N-15 edit AND C-13 EDIT NOESY files that very few c...
Posted: sri 71 / Updated: sekhar Talluri 601 Jan 15 '12 at 11:19

c13 edit noesy phasing problem

2 1 1k
answers votes views
Dear NMR Wiki'ers I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of...
Posted: sri 71 / Updated: Eugene DeRose 41 Nov 21 '11 at 14:08

Auto assignment of N15 C13 EDIT noesy experments

1 0 1k
answers votes views
Dear NMR wikiers could you please suggest any auto assignment web servers online to assign N-15 & C13- EDIT NOESY...
Updated: sri 71 Oct 17 '11 at 10:20

NOESY+WET 2D artifacts

0 0 1k
answers votes views
When i run 2d noesy with wet suppression (Hypercomplex with phase=1,2, nt=32, so phasecycle errors should be excluded)),...
Posted: Nikolay Boev 41 Sep 07 '11 at 06:07

c13 filter noesy experment for homodimer protein

1 0 1k
answers votes views
Dear NMR wikiers I would like to take c13 filter noesy experment ( this experment explore residues of the interface of...
Updated: sri 71 Jul 05 '11 at 10:56

Beginner: Help understanding 3D NOESY

1 0 1k
answers votes views
Hi, I am a person with a Computer Science background who is dealing with some NMR dataset. I am concerned with the assig...
Posted: Pyrocs 3 / Updated: Yoan Monneau 136 Jun 10 '11 at 02:22

NOESY peak signs

3 1 2k
answers votes views
I am new to NOESY, so I have a number of question about interpreting resulting 2D spectra. Mixing time is 300 ms, substa...
Posted: Dmitry Mainichev 31 / Updated: Scott Robson 146 Nov 02 '10 at 10:33

Varian Biopack pulse sequences for intermolecular NOEs

1 1 2k
answers votes views
Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the mom...
Posted: acalab 21 / Updated: Evgeny Fadeev 5571 Aug 10 '10 at 16:52

What is the more effective method of water suppression for NOESY?

2 0 3k
answers votes views
Hi I've been reading about WET water suppression scheme here where it is mentioned as superior to WATERGATE in that it a...
Posted: Evgeny Fadeev 5571 / Updated: georgeg 301 Jul 19 '10 at 08:53

Will intermediate exchange give poor half filtered NOE

1 1 2k
answers votes views
I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Posted: TomgA20 61 / Updated: Evgeny Fadeev 5571 Jul 15 '10 at 08:17

How should molecule flexibility affect NOE distance restraint calibration?

1 0 3k
answers votes views
How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Updated: Evgeny Fadeev 5571 Mar 13 '10 at 18:48

What software can do relaxation matrix analysis of NOE?

1 0 3k
answers votes views
Hello, could you suggest software to conduct proper relaxation matrix analysis of NOE? Is it available anywhere on the ...
Posted: Evgeny Fadeev 5571 / Updated: sekhar Talluri 601 Feb 13 '10 at 12:43

How to set NOESY mixing time in intermediate tumbling regime

1 0 3k
answers votes views
I need to run noesy for a monomeric peptide of about 1.5kDa at 800 MHz 1H frequency. NOE peaks are weak at 100 ms mixing...
Posted: Evgeny Fadeev 5571 / Updated: sekhar Talluri 601 Feb 13 '10 at 12:00

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 3k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Updated: Evgeny Fadeev 5571 Feb 12 '10 at 12:32
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