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0 |
648 |
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Hello, I've started using PyMol for looking at the bundles of NMR structures. You can open files one-by-one, but it's ki...
| 1 |
0 |
354 |
| answers |
votes |
views |
Hello, I'm currently working on solving a peptide NMR solution structure using NIH-XPLOR.
I find myself a lot starting ...
| 1 |
1 |
372 |
| answers |
votes |
views |
I'm gearing up for a project in which we're looking at the structure of a short peptide that should fold upon binding di...
| 1 |
1 |
363 |
| answers |
votes |
views |
Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no dis...
| 0 |
1 |
184 |
| answers |
votes |
views |
What does it mean when modelfree does not assign any model to a particular spin? Also, what should one do if more than h...
| 1 |
1 |
307 |
| answers |
votes |
views |
I have 5 different RDCs that I've extracted using simple perl scripts and Sparky .list files. I want to use XPLOR for s...
| 2 |
1 |
509 |
| answers |
votes |
views |
Hello, I'm trying to use an NMR spectrum to define/calculate a "degree of acrylation" for a PLA-PEG-PLA block copolymer ...
Updated:
juxtabo
11
Apr 11 at 10:48
| 1 |
0 |
510 |
| answers |
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views |
How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
| 3 |
2 |
439 |
| answers |
votes |
views |
Guys-has any one used fastMF program from Loria's group? I am getting an error message - "Error during input, file= 6mar...
| 3 |
0 |
706 |
| answers |
votes |
views |
Hello, what NMR experiments can detect hydrogen bonds directly?
Is there anything that can work with non-labeled sample...
| 2 |
0 |
578 |
| answers |
votes |
views |
Hello, could anyone suggest a method to determine error (maybe standard deviation or some other estimate) of a peak posi...
| 2 |
1 |
496 |
| answers |
votes |
views |
Help me in getting the books and articles related to know the basics of Pulse Sequence for Solid State NMR.
...
| 2 |
1 |
557 |
| answers |
votes |
views |
Hello. I was wondering if there are any software programs to extract RDC values, and their associated errors from proces...
| 1 |
0 |
453 |
| answers |
votes |
views |
Hello, I've started using Varian instrument at UCI two years ago and I use nmrPipe to process the data.
This one thing ...
| 3 |
2 |
462 |
| answers |
votes |
views |
Hi Guys, I noticed that the Varian file format has a bit in the status code (a short value in the 32 byte header) which ...
| 3 |
2 |
320 |
| answers |
votes |
views |
Does anyone know how, or have a resource that can assist me in processing data collected from this experiment? Thanks.
...
| 0 |
0 |
243 |
| answers |
votes |
views |
Hello, are you using pymol to analyze NMR structures? (I've been mostly using Molmol before).
Have you learned any PyMo...
| 2 |
2 |
535 |
| answers |
votes |
views |
I was wondering how to add a shaped-pulse to a standard fHSQC pulse program on a Varian?
...
| 2 |
0 |
478 |
| answers |
votes |
views |
Hi folks,
I've been using Molmol, but found significant issues when working with a non-standard structure.
For example...
| 1 |
0 |
505 |
| answers |
votes |
views |
By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry.
Also - ...
| 1 |
0 |
383 |
| answers |
votes |
views |
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
| 2 |
0 |
458 |
| answers |
votes |
views |
We do this trick with our 800 MHz Oxford Instruments-built magnet often to impress some folks:
Remove the probe and plu...
| 0 |
0 |
184 |
| answers |
votes |
views |
We've tried to measure an EPR spectrum of something in THF at -78C and could not tune the cavity on our Bruker EMX500 sp...
| 3 |
1 |
730 |
| answers |
votes |
views |
So you've analyzed your NOE data sets and have 1000+ assigned restraints, with probably inevitable errors. Going through...
| 1 |
0 |
369 |
| answers |
votes |
views |
... solving a structure of a cyclic peptide with Ornithine residue, where Orn side-chain participates in an amide bond.
...
| 1 |
0 |
305 |
| answers |
votes |
views |
Is a quantum of energy such as that in transverse RF field produced by NMR probe absorbed instantaneously by a single nu...
| 3 |
1 |
657 |
| answers |
votes |
views |
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
| 1 |
0 |
595 |
| answers |
votes |
views |
Hi I have the following problem in dqfcosy - have pretty strong antidiagonal.
I've run the same experiment before on ot...
| 0 |
2 |
230 |
| answers |
votes |
views |
Hi there! Could anyone share an example of NIH-XPLOR files for annealing of NMR structure with non-natural residues. May...
Updated:
anita
31
Jan 19 at 11:40
| 1 |
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367 |
| answers |
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Hello, I've been working through the probe performance tests and noticed that when I change pfgon='yyy' to pfgon='nyn' l...
