extract error bars from sparky S/N or deviation or both

0 0 191
answers votes views
Dear NMR wiki community, I have recorded 24 sequential 15N HSQC spectra, in an H/D exchange experiment and now, I am ha...
Updated: Maryam 1 Apr 14 at 12:57

RGA setting too high on Bruker Avance 300

2 0 263
answers votes views
I'm using Bruker Avance 300 spectrometer (Topspin processing software). When using "RGA" GR value jumps up to 3000 and m...
Posted: Avance300 1 / Updated: Kirk Marat 672 Apr 16 at 12:50

Signal intensity affected by long-range proton coupling?

1 0 260
answers votes views
Hi, I'm looking for a theoretical explanation for the effect of long-range (proton) couplings on the signal intensity of...
Updated: doodlebugs 1 Apr 14 at 06:45

195Pt experiment setup help needed

4 0 289
answers votes views
We just bought a new console (with Topspin 3.2)for our Bruker 400 and noticed that not all experiments that we previousl...
Posted: IA 1 / Updated: Kirk Marat 672 Apr 04 at 13:48

simulate 3D NOESY

1 0 182
answers votes views
Hello everyone I am using NMRPipe to simulate 3D, noise-free NOESY spectra (13C or 15N resolved) from peak lists, but I...
Updated: canavanin 17 Mar 19 at 05:17

Total t1 evolution time, TROSY, PRE distance calculation

0 0 165
answers votes views
Hi all, This is my first post to the forum and I am admittedly new to the field of protein NMR. I appreciate your patie...
Posted: Logan 1 Mar 11 at 09:04

VNMRJ Macro - wait until completion of first experiment

1 0 184
answers votes views
I am relatively new to VNMRJ, and am trying to write a macro that executes an array in experiment 1, then, once finished...
Posted: Tazmusica 1 / Updated: Bharathwaj 112 Mar 14 at 07:43

Diagnosing 1D selective NOE issues

4 0 1k
answers votes views
I'm trying to diagnose a possible issue performing a selective 1D NOE (selnogp) on a Bruker Avance II 500Mhz with TopSpi...
Updated: kyleabeauchamp 1 Mar 22 at 09:48

integration 2d noesy

0 0 580
answers votes views
I will appreciate if somebody write me how to integrate 2D NOESY spectrum in TopSpin 2.1 or 2.5! Step by step, please! I...
Posted: gugica 1 Feb 18 at 03:55

Very simple NMR question wrt resonance frequency calculation

6 0 1k
answers votes views
Hi guys, I'm stuck in attempting this question and need some help understanding how to answer it. Q.) The chemical shif...
Posted: Will Whitehouse 1 / Updated: SankarampadiAravamudhan 91 Apr 02 at 00:25

J scaling factor

1 0 698
answers votes views
Hello How to write a pulse program with J-scaling factor? When I should introduce the scaling factor? For example, how...
Posted: ghNMR 11 / Updated: Nikolay Boev 41 Feb 13 at 04:45

selective noesy

5 0 769
answers votes views
Hi, I have question for you who have Bruker instruments! On 300 MHz I worked on selective noesy pulse sequence (selnogp)...
Posted: gugica 1 / Updated: Pete Gierth 326 Feb 13 at 14:58

T2 relaxation (CPMG) measurement of 13C high resolution spectra

1 0 1k
answers votes views
Hi all, my question is how to perform a T2 measurement of 13C liquid NMR spectra in BRUKER AVIII ? What sequence to use?...
Updated: TiagoB 5 Feb 19 at 04:23

Status of Wiki

1 0 330
answers votes views
I'm just wondering what the status is of the wiki portion of this site. I receive error messages when trying to edit a ...
Posted: Kirk Marat 672 / Updated: Ulrich Haunz 101 Feb 10 at 01:38

Running a 13C{19F} spectrum on a Bruker Instrument?

1 0 407
answers votes views
My apologies in advance for the very "beginner" nature of my question : ) We are interested in examining a carbon spect...
Posted: nmrbeginner 1 / Updated: Charlie Fry 91 Jan 23 at 07:32

how to read a file generated by SPARTA+

0 0 135
answers votes views
i want to ask how to analyze a pred. file generated as a result of running SPARTA+ shift prediction software . ...
Posted: prashantiitb 1 Jan 20 at 04:34

2D ROESY-NMR with ~0 peak intensity

1 0 262
answers votes views
I have been trying to elucidate the 3D structure of oxidized glutathione (M.Wt= 612.63 g/mol) with the help of roesy dis...
Posted: Raj 1 / Updated: marcs 11 Jan 20 at 22:46

Topspin 3.2.5 queue experiment

0 0 302
answers votes views
Does anyone know how to make Topspin to queue experiments? In my recently installed Topspin somehow the queue function i...
Posted: shark 1 / Updated: MikeBrown 1 Jan 12 at 22:14

spin lock 1H spectrum

2 0 383
answers votes views
Please, if somebody wants to help me?! I need to get 1H spectrum with spin lock pulse on Bruker AV 600 instrument. Which...
Updated: gugica 1 Jan 17 at 05:06

using chenomx and missing peaks

1 0 313
answers votes views
hello, I am trying to identify and quantify of metabolites present in semen with spectra obtained from 400 MHz NMR using...
Posted: Reza mohammadi 1 / Updated: Ulrich Haunz 101 Feb 10 at 01:36

residual dipolar coupling

1 0 284
answers votes views
Hi, which aminoacid is best for calibrating acrylamide gel percentage for RDC? ...
Posted: shine a 1 / Updated: dejian 1 Dec 29 '13 at 13:53

can topspin 3.2 window version open broker fid file?

3 0 534
answers votes views
I installed the Topspin 3.2 pl 3 on windows 7, but when I am trying to open my NMR data it can only show process data fi...
Posted: shark 1 / Updated: John Hollerton 96 Jan 23 at 09:36

1-2 yrs funding to complete Ph.D. in Chemistry (Ionophores NMR Molecular Modelling)

0 0 660
answers votes views
Looking for 1-2 yrs funding to complete my Ph.D. in Chemistry. Thesis: 'A Study of Conformational Analysis and Molecular...
Posted: craigofw 1 Dec 18 '13 at 08:15

error while running humpcal

1 0 373
answers votes views
Dear all, My new avance 400 is driving me crazy at the moment ! Shims are awful although I tried the topshim 3D on sucro...
Updated: univp5 1 Dec 20 '13 at 02:44

How to calibrate pulse length for urine samples?

2 0 455
answers votes views
I recently read an article about executing a WET sequence for water suppression in urine samples. The 90°excitation puls...
Updated: brighman 1 Dec 26 '13 at 18:10

"Satellite" peaks in polypeptide HSQC

1 0 376
answers votes views
In the decoupled HSQC of a polypetide I see two small peaks right and left to the delta arginine signal. These peaks hav...
Posted: PQdotL 1 / Updated: Pete Gierth 326 Nov 29 '13 at 00:45

How to adjust the receiver gain in a deuterium oxide protein sample?

2 0 532
answers votes views
I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives...
Updated: norsouth 3 Dec 02 '13 at 11:16

PHIP experiments

0 0 201
answers votes views
Dear all! I am struggling with some density matrices in PHIP experiments. I understand why the density matrix before hyd...
Posted: Elina Lisitsin 1 Nov 28 '13 at 02:41

How to exactly set integral start and end in VNMRJ

0 0 208
answers votes views
One can roughly set the integral start and end by left clicking in the spectra. Is there a command which let you type in...
Posted: de xiao 11 Nov 27 '13 at 07:58

How can I tell if and what kind of solvent suppression was used?

2 0 1k
answers votes views
I have some older 2D HSQC 1H-15N data that was run by a NMR tech. Is there a way I can tell what kind of solvent suppre...
Posted: BrianW 1 / Updated: uabnmr1 1 Feb 26 at 07:11
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