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posted Mar 16 '10 at 13:12

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. Is there a method to measure them anyway? Will E.COSY type of experiments still work?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

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No.1 Revision

posted Mar 16 '10 at 13:13

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. Is there a method to measure them anyway? Will E.COSY type of experiments still work?be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

click to hide/show revision 3
No.2 Revision

posted Mar 16 '10 at 13:14

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons. protons (no discernible multiplet structure - even line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

click to hide/show revision 4
No.3 Revision

posted Mar 16 '10 at 14:52

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

click to hide/show revision 5
No.4 Revision

posted Mar 16 '10 at 14:53

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples?samples? Pseudo-3D experiments?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

click to hide/show revision 6
No.5 Revision

posted Mar 16 '10 at 14:53

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

Is there a way to measure scalar coupling when lines are broad?

Hello, we have a peptide where amide peaks are broad and there is no hint of observable coupling with HA protons (no discernible multiplet structure - even with line-sharpening apodization). Is there a method to measure them anyway? Will E.COSY type of experiments still be helpful to obtain the couplings?

Are there homonuclear (1H) two-dimensional experiments that allow estimation of scalar coupling by the intensity of cross-peak without resorting to heteroatom isotope labeled samples? Pseudo-3D experiments?

Are there ready pulse sequences in the standard libraries or elsewhere that can help here?

Thanks!

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