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posted Mar 08 '10 at 11:31

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5571

What NMR experiments can be used to detect hydrogen bonds?

Hello, what NMR experiments can detect hydrogen bonds directly?

Is there anything that can work with non-labeled samples at the natural abundance of isotopes?

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posted Mar 08 '10 at 11:36

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5571

What NMR experiments can be used to detect hydrogen bonds?

Hello, what NMR experiments can detect hydrogen bonds directly?

Is there anything that can work with non-labeled samples at the natural abundance of isotopes?

We have a cyclic peptide that might form a beta-sheet made of two anti-parallel strands.

The problem is that it is made of two pieces of the same sequence closed into a cycle and in such arrangement NOE's from the "middle" part (if it is indeed forming two anti-parallel beta-strands) will be hard to distinguish as being intra- or inter-strand. That's why I think direct observation of hydrogen bonds would be very helpful.

Thanks!

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