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posted Feb 26 '10 at 14:04

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a structure of nucleic acid alone.

What are (are there?) the major differences in the approaches when working on a structure of nucleic acid - in terms of choice of the NMR technique, maybe specific parameters and structure simulation?

Thank you in advance!

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expanded question

posted Mar 08 '10 at 09:45

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a structure of nucleic acid alone.

What are (are there?) the major differences in the approaches when working on a structure of nucleic acid - in terms of choice of the NMR technique, maybe specific parameters and structure simulation?

Are distance restraints alone sufficient to obtain structure of DNA or RNA?

Thank you in advance!

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