I am running BioPack on a Varian INOVA 800MHz on a newly aquired 13C15N1H sample and I have come across an issue that I was wondering if anyone could shed some light on.

When it got to the CBCAcoNH, the experiment it setup to calibrate the phi7cal is set to take ~3hours! with nt=520. I have reasonable signal (my sample is 0.8mM), so why is it doing this? Does it determine a S/N value at somepoint that maybe went awry?

I set up BioPack in the following manner:

create new experiment

load ghn_co

set parameters: TROSY='n' gzlvl1=10000 gzlvl2=9600 mag_flg='n', np=1024, tpwr=62, pwClvl=63, pwNlvl=62

set pw and tof as manually determined

Main Menu ->Setup->Proteins->Manag Probe Files=> Update Probe Files with these Parameters

Back->Calibrate->Full

Thanks for any insights!

I am running BioPack on a Varian INOVA 800MHz on a newly aquired 13C15N1H sample and I have come across an issue that I was wondering if anyone could shed some light on.

When it got to the CBCAcoNH, the experiment it setup to calibrate the phi7cal is set to take ~3hours! with nt=520. I have reasonable signal (my sample is 0.8mM), so why is it doing this? Does it determine a S/N value at somepoint that maybe went awry?

I set up BioPack in the following manner:

create new experimentexperiment, load ghn_co and set parameters:

cexp(100) jexp100 ghn_co
TROSY='n' gzlvl1=10000 gzlvl2=9600 mag_flg='n', mag_flg='n' 
np=1024, tpwr=62, pwClvl=63, pwNlvl=62
pwNlvl=62

I am running BioPack on a Varian INOVA 800MHz on a newly aquired 13C15N1H sample and I have come across an issue that I was wondering if anyone could shed some light on.

When it got to the CBCAcoNH, the experiment it setup to calibrate the phi7cal is set to take ~3hours! with nt=520. I have reasonable signal (my sample is 0.8mM), so why is it doing this? Does it determine a S/N value at somepoint that maybe went awry?

I set up BioPack in the following manner:

create new experiment, load ghn_co and set parameters:

cexp(100) jexp100 ghn_co
TROSY='n' gzlvl1=10000 gzlvl2=9600 mag_flg='n' 
np=1024, tpwr=62, pwClvl=63, pwNlvl=62

set pw and tof as manually determined

Main Menu ->Setup->Proteins->Manag Probe Files=> Update Probe Files with these Parameters

Back->Calibrate->Full

Thanks for any insights!

I am running BioPack on a Varian INOVA 800MHz on a newly aquired 13C15N1H sample and I have come across an issue that I was wondering if anyone could shed some light on.

When it got to the CBCAcoNH, the experiment it setup to calibrate the phi7cal is set to take ~3hours! with nt=520. I have reasonable signal (my sample is 0.8mM), so why is it doing this? Does it determine a S/N value at somepoint that maybe went awry?

I set up BioPack in the following manner:

create new experiment, load ghn_co and set parameters:

cexp(100) jexp100 ghn_co
TROSY='n' gzlvl1=10000 gzlvl2=9600 mag_flg='n' 
np=1024, tpwr=62, pwClvl=63, pwNlvl=62

set pw and tof as manually determined

Main Menu ->Setup->Proteins->Manag Probe Files=> Update Probe Files with these Parameters

Back->Calibrate->Full

Thanks for any insights!

I am running BioPack on a Varian INOVA 800MHz on a newly aquired 13C15N1H sample and I have come across an issue that I was wondering if anyone could shed some light on.

When it got to the CBCAcoNH, the experiment it setup to calibrate the phi7cal is set to take ~3hours! with nt=520. I have reasonable signal (my sample is 0.8mM), so why is it doing this? Does it determine a S/N value at somepoint that maybe went awry?

I set up BioPack in the following manner:

create new experiment, load ghn_co and set parameters:

cexp(100) jexp100 ghn_co
TROSY='n' gzlvl1=10000 gzlvl2=9600 mag_flg='n' 
np=1024, tpwr=62, pwClvl=63, pwNlvl=62

set pw and tof as manually determined

Main Menu ->Setup->Proteins->Manag Probe Files=> Update Probe Files with these Parameters

Back->Calibrate->Full

Thanks for any insights!