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posted Jul 20 '10 at 11:47

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

What is your test for the feasibility of protein structure determination?

How do you evaluate protein samples for the feasibility to solve 3D structure?

For example if you record a test HSQC set for a liquid state sample - what basic experimental parameters do you use and what S/N do you expect for a single peak?

What percentage of residues to you expect to be accounted for in the HSQC spectrum?

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posted Jul 20 '10 at 12:06

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

What is your test for the feasibility of protein structure determination?

How do you evaluate protein samples for the feasibility to solve 3D structure?

For example if you record a test HSQC set for a liquid state sample - what basic experimental parameters do you use and what S/N do you expect for a single peak?

What percentage of residues to you expect to be accounted for in the HSQC spectrum?

Do you have a quantitative test?

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No.2 Revision

posted Jul 20 '10 at 20:13

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

What is your test for the feasibility of protein structure determination?

How do you evaluate protein samples for the feasibility to solve 3D structure?

For example if you record a test HSQC set for a liquid state sample - what basic experimental parameters do you use and what S/N do you expect for a single peak?

What percentage of residues to you expect to be accounted for in the HSQC spectrum?spectrum? Any other considerations?

Do you have a quantitative test?

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