i like this post (click again to cancel)
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

Dear Friends,

I am working on a protein-protein complex using 700MHz VARIAN NMR . I have done HSQC perturbation studies by adding incremental ratio of protein A to B, and doing the reverse way adding protein B to A. In both the spectrum I have noticed about 20 peaks disappear or peak intensity drop in both spectrum . The total complex size I assume to be in range of 50kD. Would it be reasonable to proceed further and acquire 3D experiments for protein-protein complex structure calculation ? Any suggestions would be useful.

thanks & regards


asked Mar 14 '12 at 19:50

Arun%20Gupta's gravatar image

Arun Gupta

One Answer:
i like this answer (click again to cancel)
i dont like this answer (click again to cancel)

Are the assignment of resonances for the individual proteins known? If you are sure there is no aggregation, you could try some 3D experiments. Of course, you would want to start with HNCO.


answered Mar 15 '12 at 17:40

service%20email%20start's gravatar image

service email start

Your answer
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

powered by CNPROG