I am working on a protein-protein complex using 700MHz VARIAN NMR . I have done HSQC perturbation studies by adding incremental ratio of protein A to B, and doing the reverse way adding protein B to A. In both the spectrum I have noticed about 20 peaks disappear or peak intensity drop in both spectrum . The total complex size I assume to be in range of 50kD. Would it be reasonable to proceed further and acquire 3D experiments for protein-protein complex structure calculation ? Any suggestions would be useful.
thanks & regards
asked Mar 14 '12 at 19:50
Are the assignment of resonances for the individual proteins known? If you are sure there is no aggregation, you could try some 3D experiments. Of course, you would want to start with HNCO.
answered Mar 15 '12 at 17:40