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Hi, someone asked a similar question before, but I'd like to know:

Are there readily available varian pulse sequences that help assign aromatic side-chains? Maybe there is something like that in Biopack or elsewhere?

Thanks a bunch! M.

asked Sep 13 '10 at 11:23

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Magnethead
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updated Sep 13 '10 at 12:46


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Hi,

In VJ/BioPack you can go to the Experiments Menu/Protein Triple Resonance Experiments/Aromatics you'll find the entries for the two experiments mentioned by Evgeny. These will run the setup macros so you can run just like any other BioPack Experiment.

-George Gray

link

answered Sep 20 '10 at 10:44

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georgeg
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Hi there,

I've done a quick look at the /vnmr/psglib directory and found these:

  • hbcbcgcdceheA.c (correlates shifts CB with HD and HE)
  • hbcbcgcdhdA.c (correlates CB with HD)

Both are 2D experiments and seem to be using Biopack parameters.

Once you assign HD, HCCH-TOCSY should help you assign the remaining resonances of the aromatic side-chains (but you might have to optimize parameters of HCCH-TOCSY for the aromatics).

link

answered Sep 14 '10 at 12:02

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Evgeny Fadeev
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Is it possible to connect HD to HB using HCCH-TOCSY spectra?

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answered Nov 17 '10 at 06:57

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Dummy
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can you post it as a separate question - will HCCH-TOCSY correlate aliphatic with aromatic protons? - Evgeny Fadeev (Nov 17 '10 at 10:28)

The reason I ask - is because that way more people will see your question and it's more likely that someone answers it. Thanks. - Evgeny Fadeev (Nov 17 '10 at 10:29)

Sorry, it was because I was new to the site, then I saw regulations.. :) - Dummy (Nov 19 '10 at 06:28)

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